Lucknow (ISW) - Arsenic accumulation in rice grains is one of the serious agricultural issues in India. Researchers at Lucknow-based CSIR-National Botanical Research Institute have developed a transgenic rice by inserting a novel fungal gene, which results in reduced arsenic accumulation in rice grain.
In their latest study, researchers have cloned Arsenic methyltransferase (WaarsM) gene from a soil fungus, Westerdykell aaurantiaca , and inserted into the rice genome with the help of Agrobacterium tumefaciens, a soil bacterium which has natural ability to alter the plant's genetic makeup.
The newly developed transgenic rice along with normal rice was then treated with arsenic. Comparison of transgenic and non-transgenic rice showed that transgenic plants accumulated less arsenic in root as well as shoot compared to non-transgenic lines.
The researchers found the resultant transgenic plant acquired the potential for methylating inorganic arsenic to a variety of harmless organic species, including volatile arsenicals. This could be a potential strategy for developing transgenic rice capable of low arsenic accumulation not only in grain but also in straw and feed which are used for livestock.
Now the team is focusing on food safety test and field trials for regulatory approvals. In addition, researchers are also looking for gaps in arsenic metabolism in rice which will ultimately lead to understand arsenic uptake and metabolism in rice.
"Our study provides an understanding into arsenic transport mechanism in plants, predominantly rice grain. This knowledge can be applied to develop practices to decrease accumulation of arsenic in rice grain by molecular breeding, gene editing or transgenic approaches. It can have tremendous public health consequences," explained Dr. Debasis Chakarabarty, Scientist, Genetics and Molecular Biology at NBRI.
The research team is involved in developing biotechnological methods for reducing arsenic accumulation in rice grain. In the past, it has shown a transgenic approach in which phytochelatin synthase from Ceratophyllum demersum (an aquatic plant) was expressed in rice. Transgenic lines showed enhanced accumulation of arsenic in roots and shoot but less in grains. They also described that over expression of OsGrx_C7 (protein found in rice) enhanced tolerance to arsenite and reduced arsenite accumulation in seeds and shoots of rice. Recently, they have showed that OsPRX38 transgenics accumulate less arsenic due to high lignification in root which acts as a barrier for arsenic entry in transgenic plants.
"As large number of people is affected by arsenic toxicity, it is imperative to develop rice with lesser arsenic content and high yield. In this background, biotechnological methods such as modulating the expression of Arsenic metabolism-related genes in rice will be a fruitful and practical approach to decrease arsenic accumulation," added Dr. Chakarabarty.
Source: India Science Wire
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